Products

Direct DNA sequencing will be a routine procedure in specialized clinical laboratories within 10 years-Editorial in Clin. Chem. 41/10, 1407-1409 (1995)

The major roadblock to realizing the above prediction is the cost of preparing clinical specimens for direct DNA sequencing. LoTemp™ HiFi^® PCR has overcome this roadblock by eliminating the needs for DNA extraction and purification, thus opening the gateway to direct DNA sequencing for all clinical laboratories with a dedicated technical staff. The potential of product development is enormous. Currently, we offer the following to our customers:

(1) LoTemp™ HiFi® PCR ready-to-use Mix 1 mL x 2. Catalog #8802

Ready-to-use mix contains optimized HiFi^® DNA polymerases, buffer, Mg++, dNTPs, stabilizers, and proprietary melting reagents for purified and unpurified DNA samples. Use 20 mL for each 25 mL final PCR volume, thermocycled at 40° - 85°C. Its advantages are:

Produce more specific amplicons than any other heat-resistant enzymes
Nested PCR products used for direct automated DNA sequencing
Stable at room temperature for at least 4 weeks
Stable at 4°C for at least 10 months
Ideal for field PCR & large-scale PCR
Reduce DNA damage by LoTemp™ denaturing
Reduce in-house pipetting contamination
Just add primers and template
Do not need hot-starts

(2) β-globin PCR primer pair, optimized for specimen adequacy, amplicon

size 110 bp, forward and reverse, 150 µL each. Catalog #2003

(3) PCR product transferring glass rods, calibrated, 100 rods. Using thin glass rods to transfer PCR amplicons in nested PCR and for direct DNA sequencing reaction minimizes the risk of sample contamination. Catalog #3324

(4) HPV degenerate MY09/MY11 outer PCR primer pair, optimized for LoTemp™ PCR, amplicon size ~450 bp, 150 µL each. Catalog #3001

(5) HPV short-fragment nested PCR primer pair, optimized for LoTemp™ PCR, amplicon size 190-200 bp (flanked by GP6 and MY11 binding sites), 150 µL each. Catalog #3002

(6) HPV-16 plasmid positive control in TE buffer 100 µL. Catalog #2216

(7) HPV short-fragment DNA sequencing general primer optimized in TE buffer, 100 µL. Catalog #6007

(8) Gonococcal opa gene outer PCR primer pair, optimized for LoTemp™ PCR, amplicon size ~210-270 bp, 150 µL each. Catalog #4003

(9) Gonococcal opa gene nested PCR primer pair, optimized for LoTemp™ PCR, amplicon size ~127-130 bp, 150 µL each. Catalog #4004

(10) Neisseria gonorhoeae positive control, optimized cell digestate in TE buffer, 100 µL each. Catalog #2247

(11) Gonococcal opa gene DNA sequencing primer optimized in TE buffer, 100mL. Catalog #7008

(12) Chlamydia trachomatis cryptid plasmid outer PCR primer pair, optimized for LoTemp™ PCR, amplicon size ~290 bp, 150 µL each. Catalog #5005

(13) Chlamydia trachomatis cryptic plasmid nested PCR primer pair, optimized for LoTemp™ PCR, amplicon size ~ 160 bp, 150 µL each. Catalog #5006

(14) Chlamydia trachomatis positive DNA control, optimized cell digestate in TE buffer, 100 µL each. Catalog #2258

(15) Chlamydia trachomatis cryptic plasmid DNA sequencing primer optimized in TE buffer, 100 µL. Catalog #8009

Items 4-15 are for preparation of PCR amplicons from clinical materials to perform direct automated Sanger DNA sequencing for genotype-specific signature sequence validation of the L1 gene of HPV, and for validation of the species-specific gonococcal opa genes, and for the species-specific cryptic plasmid DNA of Chlamydia trachomatis.

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