User's Guide

LoTemp HiFi PCR mix* contains HiFi DNA polymerases optimized in proprietary matrix for PCR at temperature cycles not to exceed 85C.

For each final reaction volume of 25L, the following procedure is recommended:

Ready-to-use mix
Forward primer 
Reverse primer
Template and water 
20L
1L
1L
3L

Recommended thermocycles for general DNA amplification:

Initial: 85C 1 cycle 10 min.
Then followed by:
85C 30 sec
40C 30 sec
65C 1 min for 30-35 cycles
Final extension 65C for 5-10 minutes

*For Research Use Only; Not for use in diagnostic procedures

For more information, visit www.hifidna.com

Manufactured by HiFi DNA Tech, LLC Trumbull, CT USA

Manufacturing date
Batch No.