User's Guide

LoTemp™ HiFi^® PCR mix* contains HiFi^® DNA polymerases optimized in proprietary matrix for PCR at temperature cycles not to exceed 85ºC.

For each final reaction volume of 25µL, the following procedure is recommended:

Recommended thermocycles for general DNA amplification:

Initial: 85ºC 1 cycle 10 min.
Then followed by:
85ºC 30 sec
40ºC 30 sec
65ºC 1 min for 30-35 cycles
Final extension 65ºC for 5-10 minutes

*For Research Use Only; Not for use in diagnostic procedures

For more information, visit www.hifidna.com

Manufactured by HiFi DNA Tech, LLC Trumbull, CT USA

Manufacturing date
Batch No.