LoTemp^™ PCR^*  with HiFi^® DNA polymerase 

unloading the power of automated Sanger sequencing

in DNA testing for Pap cytology specimens

Perform DNA sequencing for HPV genotyping beyond a reasonable doubt -  BMC Clin Path 2009, 9:3 (22 May 2009).   

No-false positive Lyme disease DNA testing;

Validate the DNA of Neisseria gonorrhoeae opa genes beyond a reasonable doubt;

Validate the DNA of Chlamydia trachomatis cryptic plasmid beyond a reasonable doubt;

Using the GenBank database as the standard for validating sequence algorithms;

On 5% of a liquid-based Pap cytology specimen without need of DNA extraction.

These are cutting-edge molecular tests developed by pathologists/ cytotechnologists for pathologists/cytotechnologists to serve gynecologists and their patients at sustainable cost.  

Application of LoTemp® PCR/DNA sequencing recent publications:

1. Detection of human papillomavirus L1 gene DNA fragments in postmortem blood and spleen after Gardasil® vaccination-A case report

2. Detection of human papillomavirus (HPV) L1 gene DNA possibly bound to particulate aluminum adjuvant in the HPV vaccine Gardasil®

3. Guidelines for the use of molecular tests for the detection and genotyping of human papillomavirus from clinical specimens

4. Early Lyme disease with spirochetemia - diagnosed by DNA sequencing.

* Lee SH, Vigliotti VS, Vigliotti JS, Pappu S, Routine human papillomavirus genotyping by DNA sequencing in community hospital laboratories. Infect Agent Cancer 2007:2:11.

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